Review



2sa mutant constructs  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Addgene inc 2sa mutant constructs
    Notch1 intracellular domain induces ubiquitination and degradation of the Snail protein . (A) Hep3B cells were transfected with Myc-Notch1 intracellular domain (NICD) and/or hemagglutinin (HA)-Snail, then treated with MG132 for 6 hours. Expression of Myc-NICD and HA-Snail was then analyzed by immunoblotting. (B) HA-Snail- or HA-Snail/Myc-NICD-transfected Hep3B cells were treated with 20 μM cycloheximide for different time intervals, then evaluated for Snail protein levels using immunoblot analysis. (C) 293T cells were transfected with Myc-NICD, SFB-Snail and/or HA-tagged ubiquitin, then treated with MG132 for 6 hours. Snail protein was immunoprecipitated using an anti-Flag antibody. Cell lysates were analyzed by immunoblotting with the indicated antibodies. (D) Hep3B cells were transfected with Myc-NICD, HA-Snail and MDM2; coimmunoprecipitated using anti-Myc and anti-MDM2 antibodies, respectively; and evaluated by immunoblot analysis. Immunoglobulin G served as a negative control. (E) Hep3B cells were transfected with Myc-NICD, HA-Snail and/or MDM2 siRNA and evaluated for the expression of Myc-NICD, HA-Snail and MDM2 using immunoblot analysis. (F) Hep3B cells were transfected with Myc-NICD and/or Flag-Snail wild-type (WT) or <t>2SA</t> mutant. The expression of Myc-NICD and Flag-Snail was then analyzed by immunoblotting. β-actin was used as an internal control.
    2sa Mutant Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2sa mutant constructs/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    2sa mutant constructs - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Notch1 binds and induces degradation of Snail in hepatocellular carcinoma"

    Article Title: Notch1 binds and induces degradation of Snail in hepatocellular carcinoma

    Journal: BMC Biology

    doi: 10.1186/1741-7007-9-83

    Notch1 intracellular domain induces ubiquitination and degradation of the Snail protein . (A) Hep3B cells were transfected with Myc-Notch1 intracellular domain (NICD) and/or hemagglutinin (HA)-Snail, then treated with MG132 for 6 hours. Expression of Myc-NICD and HA-Snail was then analyzed by immunoblotting. (B) HA-Snail- or HA-Snail/Myc-NICD-transfected Hep3B cells were treated with 20 μM cycloheximide for different time intervals, then evaluated for Snail protein levels using immunoblot analysis. (C) 293T cells were transfected with Myc-NICD, SFB-Snail and/or HA-tagged ubiquitin, then treated with MG132 for 6 hours. Snail protein was immunoprecipitated using an anti-Flag antibody. Cell lysates were analyzed by immunoblotting with the indicated antibodies. (D) Hep3B cells were transfected with Myc-NICD, HA-Snail and MDM2; coimmunoprecipitated using anti-Myc and anti-MDM2 antibodies, respectively; and evaluated by immunoblot analysis. Immunoglobulin G served as a negative control. (E) Hep3B cells were transfected with Myc-NICD, HA-Snail and/or MDM2 siRNA and evaluated for the expression of Myc-NICD, HA-Snail and MDM2 using immunoblot analysis. (F) Hep3B cells were transfected with Myc-NICD and/or Flag-Snail wild-type (WT) or 2SA mutant. The expression of Myc-NICD and Flag-Snail was then analyzed by immunoblotting. β-actin was used as an internal control.
    Figure Legend Snippet: Notch1 intracellular domain induces ubiquitination and degradation of the Snail protein . (A) Hep3B cells were transfected with Myc-Notch1 intracellular domain (NICD) and/or hemagglutinin (HA)-Snail, then treated with MG132 for 6 hours. Expression of Myc-NICD and HA-Snail was then analyzed by immunoblotting. (B) HA-Snail- or HA-Snail/Myc-NICD-transfected Hep3B cells were treated with 20 μM cycloheximide for different time intervals, then evaluated for Snail protein levels using immunoblot analysis. (C) 293T cells were transfected with Myc-NICD, SFB-Snail and/or HA-tagged ubiquitin, then treated with MG132 for 6 hours. Snail protein was immunoprecipitated using an anti-Flag antibody. Cell lysates were analyzed by immunoblotting with the indicated antibodies. (D) Hep3B cells were transfected with Myc-NICD, HA-Snail and MDM2; coimmunoprecipitated using anti-Myc and anti-MDM2 antibodies, respectively; and evaluated by immunoblot analysis. Immunoglobulin G served as a negative control. (E) Hep3B cells were transfected with Myc-NICD, HA-Snail and/or MDM2 siRNA and evaluated for the expression of Myc-NICD, HA-Snail and MDM2 using immunoblot analysis. (F) Hep3B cells were transfected with Myc-NICD and/or Flag-Snail wild-type (WT) or 2SA mutant. The expression of Myc-NICD and Flag-Snail was then analyzed by immunoblotting. β-actin was used as an internal control.

    Techniques Used: Transfection, Expressing, Western Blot, Immunoprecipitation, Negative Control, Mutagenesis



    Similar Products

    2sa mutant constructs  (Addgene inc)
    90
    Addgene inc 2sa mutant constructs
    Notch1 intracellular domain induces ubiquitination and degradation of the Snail protein . (A) Hep3B cells were transfected with Myc-Notch1 intracellular domain (NICD) and/or hemagglutinin (HA)-Snail, then treated with MG132 for 6 hours. Expression of Myc-NICD and HA-Snail was then analyzed by immunoblotting. (B) HA-Snail- or HA-Snail/Myc-NICD-transfected Hep3B cells were treated with 20 μM cycloheximide for different time intervals, then evaluated for Snail protein levels using immunoblot analysis. (C) 293T cells were transfected with Myc-NICD, SFB-Snail and/or HA-tagged ubiquitin, then treated with MG132 for 6 hours. Snail protein was immunoprecipitated using an anti-Flag antibody. Cell lysates were analyzed by immunoblotting with the indicated antibodies. (D) Hep3B cells were transfected with Myc-NICD, HA-Snail and MDM2; coimmunoprecipitated using anti-Myc and anti-MDM2 antibodies, respectively; and evaluated by immunoblot analysis. Immunoglobulin G served as a negative control. (E) Hep3B cells were transfected with Myc-NICD, HA-Snail and/or MDM2 siRNA and evaluated for the expression of Myc-NICD, HA-Snail and MDM2 using immunoblot analysis. (F) Hep3B cells were transfected with Myc-NICD and/or Flag-Snail wild-type (WT) or <t>2SA</t> mutant. The expression of Myc-NICD and Flag-Snail was then analyzed by immunoblotting. β-actin was used as an internal control.
    2sa Mutant Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2sa mutant constructs/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    2sa mutant constructs - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier
    2sa mutant construct  (Addgene inc)
    90
    Addgene inc 2sa mutant construct
    Notch1 intracellular domain induces ubiquitination and degradation of the Snail protein . (A) Hep3B cells were transfected with Myc-Notch1 intracellular domain (NICD) and/or hemagglutinin (HA)-Snail, then treated with MG132 for 6 hours. Expression of Myc-NICD and HA-Snail was then analyzed by immunoblotting. (B) HA-Snail- or HA-Snail/Myc-NICD-transfected Hep3B cells were treated with 20 μM cycloheximide for different time intervals, then evaluated for Snail protein levels using immunoblot analysis. (C) 293T cells were transfected with Myc-NICD, SFB-Snail and/or HA-tagged ubiquitin, then treated with MG132 for 6 hours. Snail protein was immunoprecipitated using an anti-Flag antibody. Cell lysates were analyzed by immunoblotting with the indicated antibodies. (D) Hep3B cells were transfected with Myc-NICD, HA-Snail and MDM2; coimmunoprecipitated using anti-Myc and anti-MDM2 antibodies, respectively; and evaluated by immunoblot analysis. Immunoglobulin G served as a negative control. (E) Hep3B cells were transfected with Myc-NICD, HA-Snail and/or MDM2 siRNA and evaluated for the expression of Myc-NICD, HA-Snail and MDM2 using immunoblot analysis. (F) Hep3B cells were transfected with Myc-NICD and/or Flag-Snail wild-type (WT) or <t>2SA</t> mutant. The expression of Myc-NICD and Flag-Snail was then analyzed by immunoblotting. β-actin was used as an internal control.
    2sa Mutant Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2sa mutant construct/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    2sa mutant construct - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Notch1 intracellular domain induces ubiquitination and degradation of the Snail protein . (A) Hep3B cells were transfected with Myc-Notch1 intracellular domain (NICD) and/or hemagglutinin (HA)-Snail, then treated with MG132 for 6 hours. Expression of Myc-NICD and HA-Snail was then analyzed by immunoblotting. (B) HA-Snail- or HA-Snail/Myc-NICD-transfected Hep3B cells were treated with 20 μM cycloheximide for different time intervals, then evaluated for Snail protein levels using immunoblot analysis. (C) 293T cells were transfected with Myc-NICD, SFB-Snail and/or HA-tagged ubiquitin, then treated with MG132 for 6 hours. Snail protein was immunoprecipitated using an anti-Flag antibody. Cell lysates were analyzed by immunoblotting with the indicated antibodies. (D) Hep3B cells were transfected with Myc-NICD, HA-Snail and MDM2; coimmunoprecipitated using anti-Myc and anti-MDM2 antibodies, respectively; and evaluated by immunoblot analysis. Immunoglobulin G served as a negative control. (E) Hep3B cells were transfected with Myc-NICD, HA-Snail and/or MDM2 siRNA and evaluated for the expression of Myc-NICD, HA-Snail and MDM2 using immunoblot analysis. (F) Hep3B cells were transfected with Myc-NICD and/or Flag-Snail wild-type (WT) or 2SA mutant. The expression of Myc-NICD and Flag-Snail was then analyzed by immunoblotting. β-actin was used as an internal control.

    Journal: BMC Biology

    Article Title: Notch1 binds and induces degradation of Snail in hepatocellular carcinoma

    doi: 10.1186/1741-7007-9-83

    Figure Lengend Snippet: Notch1 intracellular domain induces ubiquitination and degradation of the Snail protein . (A) Hep3B cells were transfected with Myc-Notch1 intracellular domain (NICD) and/or hemagglutinin (HA)-Snail, then treated with MG132 for 6 hours. Expression of Myc-NICD and HA-Snail was then analyzed by immunoblotting. (B) HA-Snail- or HA-Snail/Myc-NICD-transfected Hep3B cells were treated with 20 μM cycloheximide for different time intervals, then evaluated for Snail protein levels using immunoblot analysis. (C) 293T cells were transfected with Myc-NICD, SFB-Snail and/or HA-tagged ubiquitin, then treated with MG132 for 6 hours. Snail protein was immunoprecipitated using an anti-Flag antibody. Cell lysates were analyzed by immunoblotting with the indicated antibodies. (D) Hep3B cells were transfected with Myc-NICD, HA-Snail and MDM2; coimmunoprecipitated using anti-Myc and anti-MDM2 antibodies, respectively; and evaluated by immunoblot analysis. Immunoglobulin G served as a negative control. (E) Hep3B cells were transfected with Myc-NICD, HA-Snail and/or MDM2 siRNA and evaluated for the expression of Myc-NICD, HA-Snail and MDM2 using immunoblot analysis. (F) Hep3B cells were transfected with Myc-NICD and/or Flag-Snail wild-type (WT) or 2SA mutant. The expression of Myc-NICD and Flag-Snail was then analyzed by immunoblotting. β-actin was used as an internal control.

    Article Snippet: Flag-Snail WT and 2SA mutant constructs were obtained from Addgene, Inc (Cambridge, MA, USA).

    Techniques: Transfection, Expressing, Western Blot, Immunoprecipitation, Negative Control, Mutagenesis